Anti-GATA-1 Antibody (N1) recommended for detection of GATA-1 of mouse, rat and human origin by WB, IP and IF

Anti-GATA-1 Antibody (N1): sc-266

Anti-GATA-1 Antibody (N1) is rated 5.0 out of 5 by 10.
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Datasheets

    • GATA-1 Antibody (N1) is a rat monoclonal IgG2a, cited in 66 publications, provided at 200 µg/ml
    • raised against bacterially expressed murine GATA-1
    • Anti-GATA-1 Antibody (N1) is recommended for detection of GATA-1 of mouse, rat and human origin by WB, IP and IF; inhibits DNA binding in gel shift studies
    • Anti-GATA-1 Antibody (N1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-266 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GATA-1 (N1): sc-266.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GATA-1 Antibody (N1). This work is in progress.
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Does GATA-1 (N1) sc-266 bind to N-terminal or C-terminal of GATA1? I want to detect C-terminal only. Thank you

Asked by: Double T
Thank you for your questin. GATA-1 Antibody (N1): sc-266 was raised against bacterially expressed murine GATA-1. No epitope mapping of this antibody has so far been performed, so we are not able to say whether it recognizes the N- or C-terminus or an internal region of the protein. Feel free to contact our Technical Service Department for more information on how to receive a free 10 µg sample of GATA-1 (N1): sc-266 if you would like to test the antibody.
Answered by: Tech Support Europe
Date published: 2020-06-29

When should the TransCruz reagent sc-266 X be used instead of the standard form sc-266?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Tech Service
Date published: 2017-01-31
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Rated 5 out of 5 by from 免疫組織化学で良好な結果 免疫蛍光染色により精巣におけるセルトリ細胞のGATA-1を検出できた。 さらに、特異性も非常に高く優れた抗体である。
Date published: 2021-01-29
Rated 5 out of 5 by from Nice band We used this antibody for a western blot and got a very clean band
Date published: 2016-12-23
Rated 5 out of 5 by from This antibody worked well for IP This antibody worked well for IP, published with Cos, HPCs (human) and mouse lymphoma cells. -SCBT Publication Review
Date published: 2015-04-20
Rated 5 out of 5 by from This GATA This GATA-1 antiobody (N1) was useful in immunohistochemistry using human hematopoietic cells. -SCBT Publication Review
Date published: 2015-04-16
Rated 5 out of 5 by from Publishable data obtained using product sc Publishable data obtained using product sc-266 in immunohistochemistry on formalin fixed, paraffin embedded (FFPE) mouse gonads. -SCBT Publication Review
Date published: 2015-04-06
Rated 5 out of 5 by from Works well in Western Blot using human hematopoietic, HPCs, erythroid, K-562, MEL, UT-7, Jurkat, HeLa, UT-7 5.3, U-937, HL-60 HEL and TF1 cell lines. -SCBT Publication Review
Date published: 2015-04-03
Rated 5 out of 5 by from Human MEL and umbelical vein endothelial Human MEL and umbelical vein endothelial cells were used in ChIP application successfully and results published. -SCBT Publication Review
Date published: 2015-02-20
Rated 5 out of 5 by from Antibody was used successfully in EMSA using Antibody was used successfully in EMSA using mouse C19 MEL and human Jurkat, HeLa, K-562, ovarian and breast cells. -SCBT Publication Review
Date published: 2015-01-25
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