Ganglioside GD3 Antibody (MB3.6)recommended for detection of Ganglioside GD3 of human origin by IF and FCM

Ganglioside GD3 Antibody (MB3.6): sc-33685

Ganglioside GD3 Antibody (MB3.6) is rated 3.0 out of 5 by 2.
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Datasheets

    • Ganglioside GD3 Antibody (MB3.6) is a mouse monoclonal mouse IgG3 κ (kappa light chain) provided at 200 µg/ml
    • raised against a human melanoma cell line
    • recommended for detection of Ganglioside GD3 of human origin by IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ganglioside GD3 (MB3.6): sc-33685.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can Ganglioside GD3 (MB3.6): sc-33685 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Ganglioside GD3 (MB3.6): sc-33685 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 3 out of 5 by from Produced nice immunofluorescence cytoplasmic Produced nice immunofluorescence cytoplasmic staining in methanol-fixed SK-MEL-28 cells. -SCBT QC
Date published: 2015-04-10
Rated 3 out of 5 by from Good indirect FCM data of SK Good indirect FCM data of SK-MEL-28 cells stained with GD3 . -SCBT QC
Date published: 2014-07-18
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