Ganglioside GD2 Antibody (14G2a)recommended for detection of Ganglioside GD2 of human origin by IF, IHC(P) and FCM

Ganglioside GD2 Antibody (14G2a): sc-53831

Ganglioside GD2 Antibody (14G2a) is rated 4.4 out of 5 by 5.
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Datasheets

    • Ganglioside GD2 Antibody (14G2a) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against neuroectodermal tumor cells of human origin
    • Ganglioside GD2 Antibody (14G2a) is recommended for detection of Ganglioside GD2 of human origin by IF, IHC(P) and FCM
    • Ganglioside GD2 Antibody (14G2a) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ganglioside GD2 (14G2a): sc-53831.
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Hi, Just wondering whether you have the sequence for the variable region of GD-2 antibody or can you provide the hybridoma for sequencing. Thank you. Jue

Asked by: Jack cells
Thank you for your question. This antibody was raised against neuroectodermal tumor cells of human origin. The exact epitope has not been epitope mapped. We are not able to provide the hybridoma for sequencing.
Answered by: Tech Service
Date published: 2019-09-04

Dose Ganglioside GD2 (14G2a) has certificated of anaylsis for QC department for validation use?

Asked by: JR Chen
Thank you for your question. We do not provide a certificate of analysis for any of our research antibodies. Please contact our Technical Service Department for any specific questions about how the antibody has been tested. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2018-08-27

From the description of the product, I understand that the 14(G2a) antibody is provided as the kappa light chain of the antibody. How can the kappa light chain recognise the GD2 sugar without the heavy chain? Many thanks

Asked by: DMax
Thank you for the question. The Ganglioside GD2 (14G2a) is a full mouse IgG2a antibody. We characterized the type of light chain because instead of conventional secondary antibodies against full-IgG we instead use a light-chain-specific binding protein which tends to produce cleaner results compared to conventional secondary antibodies. In this case we suggest using m-IgGκappa-specific BP-HRP: sc-516102 for the detection of Ganglioside GD2 (14G2a) sc-53831.
Answered by: Technical Support Europe
Date published: 2018-01-10

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-22

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Cweed
When attempting a double or triple stain with mouse monoclonal antibodies, we recommend using a directly conjugated primary antibody. This product, sc-53831, is available conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2016-12-22
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Rated 2 out of 5 by from Unzufriedenes Ergebnis in der IHC Antikörper vor etwa 3 Wochen bekommen. Schnelle Lieferung. Antikörper hat nicht die gewünschte Färbung an Paraffinschnitten geziegt
Date published: 2018-04-16
Rated 5 out of 5 by from nicely stains cultured neuroblastoma cells Immunofluorescence analysis of cultured human neuroblastoma cells fixed with paraformaldehyde. Samples were incubated with sc-53831 GD2 (14G2a) antibody (1:200) in 1% BSA + 1% serum for 1 h. An anti-mouse AlexaFluor®-488 was used as secondary antibody (1:400).
Date published: 2018-02-13
Rated 5 out of 5 by from specificity and clean Distinct band, clean background observed by WB using Ganglioside GD2 antibody.
Date published: 2017-05-07
Rated 5 out of 5 by from Published antibody Ganglioside GD2 Antibody (14G2a) has been cited in several publications
Date published: 2017-01-12
Rated 5 out of 5 by from This antibody was used in a publication for This antibody was used in a publication for FCM on human cell culture sample - SCBT Publication Review
Date published: 2015-03-23
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