Anti-gamma Tubulin Antibody (D-10) recommended for detection of γ Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-γ Tubulin Antibody (D-10): sc-17788

Anti-γ Tubulin Antibody (D-10) is rated 4.3 out of 5 by 6.
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Datasheets

    • γ Tubulin Antibody (D-10) is a mouse monoclonal IgG2b κ, cited in 32 publications, provided at 200 µg/ml
    • raised against amino acids 269-451 mapping at the C-terminus of γ Tubulin of human origin
    • Anti-gamma Tubulin Antibody (D-10) is recommended for detection of γ Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Anti-gamma Tubulin Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of γ Tubulin (D-10): sc-17788.

Can γ Tubulin (D-10): sc-17788 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenni
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, γ Tubulin (D-10): sc-17788 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from 蛍光免疫染色 4%PFA固定したラット組織の凍結切片と、賦活化処理したパラフィン切片に対して蛍光免疫染色を行いましたが、シグナルが検出できませんでした。
Date published: 2018-11-26
Rated 5 out of 5 by from nice western blot results We used this antibody in a western blot and produced great band results at the correct molecular weight
Date published: 2017-01-25
Rated 5 out of 5 by from Good cytoplasmic staining with paraffin Good cytoplasmic staining with paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-03-16
Rated 5 out of 5 by from Produced good quality flow cytometry data Produced good quality flow cytometry data with HeLa samples -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Good antibody for Western Good antibody for Western, strong gamma tubulin bands detected in HeLa and A-431 samples. -SCBT QC
Date published: 2015-01-08
Rated 5 out of 5 by from Good IF data with human HeLa samples Good IF data with human HeLa samples. -SCBT QC
Date published: 2014-09-24
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