Anti-gamma Tubulin Antibody (C-11) recommended for detection of γ Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-γ Tubulin Antibody (C-11): sc-17787

Anti-γ Tubulin Antibody (C-11) is rated 4.6 out of 5 by 17.
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Datasheets

    • Anti-γ Tubulin Antibody (C-11) is a mouse monoclonal IgG2a κ γ Tubulin antibody, cited in 78 publications, provided at 200 µg/ml
    • raised against amino acids 269-451 of γ Tubulin of human origin
    • Anti-gamma Tubulin Antibody (C-11) is recommended for detection of γ Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-gamma Tubulin Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of γ Tubulin (C-11): sc-17787.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for γ Tubulin Antibody (C-11) for WB and IHC(P) applications. These reagents are now offered in bundles with γ Tubulin Antibody (C-11) (see ordering information below).

Can this mouse monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: sweetums
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, our gamma Tubulin sc-17787 is available in a variety of direct conjugations, such as HRP, PE, FITC, AlexaFluor 488 and AlexaFluor 647.
Answered by: Technical Support 12
Date published: 2017-01-12

Is there any data showing this antibody is specific to gamma Tubulin?

Asked by: sweetums
Thank you for your question. We have immunofluorescence data with methanol fixed HeLa cells treated with siRNA to show knockdown of signal, confirming specificity to gamma Tubulin.
Answered by: Technical Support 12
Date published: 2017-01-12
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Rated 4 out of 5 by from Good I used this wnatibody for western blot. I diluted it at 1:1000 and it works well.
Date published: 2021-04-16
Rated 5 out of 5 by from Specific antibody for immunostaining Worked well for visualization of centrosomes (Confocal microscopy): Specific and very low background
Date published: 2021-03-23
Rated 5 out of 5 by from 蛍光免疫染色 4%PFA固定したラット組織の凍結切片と、賦活化処理したパラフィン切片に対して蛍光免疫染色を行いました。どちらの条件でも、特異的なシグナルが得られました。また、希釈倍率も500~1000倍で使用でき、満足しています。
Date published: 2018-11-26
Rated 5 out of 5 by from funciona bien Funciona bien en inmunofluorescencia con dilucion 1:100
Date published: 2018-04-16
Rated 5 out of 5 by from Very well results This antibody generates a clear and strong signal at the predicted position. Very good.
Date published: 2018-01-25
Rated 5 out of 5 by from Good antibody Nice antibody working in IF, WB for mouse cell line
Date published: 2017-10-12
Rated 5 out of 5 by from Tested for IF: very good staining Tested at 2 ug/ml on PFA/MeOH fix/perm primary cells. very good results
Date published: 2017-10-05
Rated 5 out of 5 by from This antibody is nice to do IF I did IF using this antibody at 1:200, the signal was good.
Date published: 2017-07-24
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