Anti-G alpha s/olf Antibody (E-7) recommended for detection of Gα s1-4 and Gα olf of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Gα s/olf Antibody (E-7): sc-55546

Anti-Gα s/olf Antibody (E-7) is rated 4.8 out of 5 by 5.
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Datasheets

    • Gα s/olf Antibody (E-7) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 82-381 mapping at the C-terminus of Gα s/olf of human origin
    • recommended for detection of Gα s1-4 and Gα olf of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See Gα s/olf (A-5): sc-55545 for Gα s/olf antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Gα s/olf Antibody (E-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Gα s/olf Antibody (E-7) (see ordering information below).
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I am using Gα s/olf (E-7): sc-55546 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from obvious It is quite obvious that the function of the heterotrimeric G-protein is to specifically detect extracellular stimuli in each of a wide range of receptors that transmit information from cell surface receptors to intracellular effectors, but we can monitor To less effectors for the production of one or more intracellular messengers.
Date published: 2017-02-08
Rated 5 out of 5 by from Nuclear Nuclear, cytoplasmic and membrane staining observed of urothelial cells observed via immunoperoxidase staining of formalin fixed, paraffin embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by from Showed high affinity/sensitivity antibodies Showed high affinity/sensitivity antibodies for the GPCR signaling/regulatory pathway in HEK293 cells.
Date published: 2015-03-30
Rated 4 out of 5 by from Crisp bands (light background) observed at Crisp bands (light background) observed at the corresponding molecular weights for G s/olf proteins by Western Blot using SK-N-MC and T98G whole cell lysates. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Strong cytoplasmic and membrane localization Strong cytoplasmic and membrane localization by immunofluorescence using methanol fixed HeLa cells. -SCBT QC
Date published: 2013-11-27
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