Anti-G alpha s/olf Antibody (A-5) recommended for detection of Gα s1-4 and Gα olf of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Gα s/olf Antibody (A-5): sc-55545

Anti-Gα s/olf Antibody (A-5) is rated 5.0 out of 5 by 9.
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Datasheets

    • Gα s/olf Antibody (A-5) is a mouse monoclonal IgG1 κ, cited in 21 publications, provided at 200 µg/ml
    • raised against amino acids 82-381 mapping at the C-terminus of Gα s/olf of human origin
    • Anti-G alpha s/olf Antibody (A-5) is recommended for detection of Gα s1-4 and Gα olf of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-G alpha s/olf Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Gα s/olf (A-5): sc-55545.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Gα s/olf Antibody (A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Gα s/olf Antibody (A-5) (see ordering information below).
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What is meant by raised against Gas/olf? Is it that the peptide sequence used is identical between two different Ga isoforms and thus the antibody targets the same epitope?

Asked by: Brian29
For more information Please Contact Technical Service.
Answered by: Tech Sales
Date published: 2021-10-26

I received this antibody (sc-55545) yesterday, and it just says that it has to be kept in 4°C. Do we need to add something inside the tube (like glycerol as for some other antibodies) to better conserve the antibody or is it fine like it is at 4°C ?

Asked by: scnath
Thank you for your question. There is no need to add anything to the antibody, please just store it at 4˚C and use as directed. It will be fine if stored properly at 4˚C.
Answered by: Tech Service
Date published: 2019-10-03

I had a aliquot of this antibody (sc55545) lot #G1116 and I want to know whats is it expiration date.

Asked by: VictorX
Thank you for your question. We do not characterize expiration dates for our research antibodies. They are warrantied for one year from the date of purchase. However, when stored properly at 4C they can continue to be viable for much longer than the one year warranty period.
Answered by: Technical Support
Date published: 2019-05-01

Can Gα s/olf (A-5): sc-55545 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Gα s/olf (A-5): sc-55545 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from G-α s/olf 抗体 (A-5)抗体真不错,效果很好! 我一个月之前买的这个抗体,当时是根据价格选的比较经济实惠,没想到做的实验效果非常好,老师非常满意,真的不错!
Date published: 2017-11-03
Rated 5 out of 5 by from Perfect for olfactory epithelium Antibody works perfect with frozen section of mouse olfactory epithelium. Tissue was fixed with PFA. Antibody gives very specific signals in ciliary part of olfactory epithelium.
Date published: 2017-10-05
Rated 5 out of 5 by from 抗体很好用的 用的小样申请装抗体,效果还不错,WB显色没有那么亮,但完全符合要求,推荐使用,做免疫组化的话,效果还不确定
Date published: 2017-08-29
Rated 5 out of 5 by from Great for WB With this antibody using 1:1000 for Western Blot analysis, antibody is very stable and shows no non-specific bands or background for WB.
Date published: 2017-05-07
Rated 5 out of 5 by from western blot Used for western blot with D-5 with murine kidney samples, worked well in western blotting.
Date published: 2017-02-25
Rated 5 out of 5 by from Bands observed at the expected molecular Bands observed at the expected molecular weight for G s/olf proteins by Western Blot using SK-N-MC and T98G whole cell lysates. -SCBT QC
Date published: 2015-04-23
Rated 5 out of 5 by from Nuclear Nuclear, cytoplasmic and membrane staining of urothelial cells observed by immunoperoxidase staining of formalin fixed, paraffin embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-04-22
Rated 5 out of 5 by from Publishable data obtained by immunohistochemistry Publishable data obtained by immunohistochemistry using african lungfish nasal sac and brain. -SCBT Publication Review
Date published: 2015-01-20
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