Anti-G alpha i-3 Antibody (H-7) recommended for detection of Gα i-1 and Gα i-3 of mouse, rat, human and origin by WB, IP, IF and ELISA

Anti-Gα i-3 Antibody (H-7): sc-365422

Anti-Gα i-3 Antibody (H-7) is rated 5.0 out of 5 by 8.
  • y_2022, m_1, d_26, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_en_US, sid_365422, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 136ms
  • REVIEWS, PRODUCT
loading
5
8
4
0
3
0
2
0
1
0

Datasheets

    • Gα i-3 Antibody (H-7) is a mouse monoclonal IgG3 κ, cited in 7 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 339-354 at the C-terminus of Gα i-3 of rat origin
    • recommended for detection of Gα i-1 and Gα i-3 of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Gα i-3 (H-7): sc-365422.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Gα i-3 Antibody (H-7) for WB applications. This reagent is now offered in a bundle with Gα i-3 Antibody (H-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
loading

Does this antibody work well in Raw 264.7 and Jurkat cell lines?

Asked by: little star123
Thank you for your question. We do not currently have any data with this antibody in either of these cell lines.
Answered by: Technical Support
Date published: 2018-11-30

How likely is it that this antibody will show a cross reaction to Gnai2 since they are similar in structure? Was this tested? How similar is the immunogen the antibody was raised against to Gnai2? Thanks in advance!

Asked by: Gwen
Thank you for your question. This mouse monoclonal antibody, Gα i-3 (H-7): sc-365422, is specific for an epitope mapping between amino acids 339-354 at the C-terminus of Gα i-3 of rat origin. This region does share significant identity (80%) with the Gα i-2 protein, so there is potential of cross-reactivity. Unfortunately, this cross-reactivity has not yet been tested. For a limited time, we are offering a free sample of this antibody. Please contact our Technical Service Department if you would like to request a sample. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-18

What application is the blocking peptide sc-365422 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2022, m_1, d_26, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_en_US, sid_365422, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 109ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from great for western blot This antibody worked great for western blot, I get clear bands by using 1:600.
Date published: 2017-02-25
Rated 5 out of 5 by from Antibody is really great. Antibody is really great,it is being used for blotting or staining.
Date published: 2017-02-13
Rated 5 out of 5 by from Great antibody for WB This antibody is really great. In the experiment, the analysis results of Western blot were very strong and clear, and the baseband is perfect.I suggest dilution this antibody at 1:200.
Date published: 2017-01-26
Rated 5 out of 5 by from Specific and clear G alpha i-3 Antibody (H-7) produced clean and specific bands in multiple samples, and IF results were excellent
Date published: 2017-01-25
Rated 5 out of 5 by from Good Good for western blot with human cell lines, worked wonderfully in our WB, Used at 1:1000.
Date published: 2017-01-12
Rated 5 out of 5 by from Good specificity in WB in HeLa whole cell lysate Good specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-26
Rated 5 out of 5 by from Strong cytoplasmic localization in formalin Strong cytoplasmic localization in formalin-fixed HepG2 cells in IF. -SCBT QC
Date published: 2015-01-12
Rated 5 out of 5 by from Good staining in IHC in retina and the outer Good staining in IHC in retina and the outer ears of mice. -SCBT Publication Review
Date published: 2013-08-20
  • y_2022, m_1, d_26, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_en_US, sid_365422, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT