Anti-G alpha 12 Antibody (E-12) recommended for detection of Gα 12 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Gα 12 Antibody (E-12): sc-515445

Anti-Gα 12 Antibody (E-12) is rated 3.0 out of 5 by 3.
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Datasheets

    • Anti-Gα 12 Antibody (E-12) is a mouse monoclonal IgG1 κ Gα 12 antibody, cited in 11 publications, provided at 200 µg/ml
    • raised against amino acids 89-300 mapping within an internal region of Gα 12 of human origin
    • Anti-G alpha 12 Antibody (E-12) is recommended for detection of Gα 12 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-G alpha 12 Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Gα 12 (E-12): sc-515445.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Gα 12 Antibody (E-12) for WB applications. These reagents are now offered in bundles with Gα 12 Antibody (E-12) (see ordering information below).
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I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-515445: Gå 12 (E-12) mouse monoclonal antibody will work?

Asked by: Randy McDonald
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06

Hello I was wondering whether the sc-515445 G12 antibody has been tested for immunohistochemistry (IHC)? I see that this technique is not in the recommended applications list. Thanks Vernita

Asked by: Vernita
Thank you for your question. This antibody has not been tested for IHC on formalin fixed paraffin embedded tissue sections, so it is not warrantied for that use. It may work, but we have not tested it in house yest so we can not warranty it. However, we do warranty it for IF on cells and IHC on fresh frozen tissue section fixed in methanol or acetone. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2016-11-18
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Rated 1 out of 5 by from High WB background & poor customer service We've tested this in western blot, using HepG2, RAW 264.7 (which should express Ga12), and THP-1 cell lines at 1:500, and in KG-1 and HL-60 at 1:200. In both cases we had very high background and are not confident even in the RAW line that we have a positive band. We contacted Santa Cruz twice to discuss troubleshooting options and have not received any replies.
Date published: 2022-03-10
Rated 3 out of 5 by from High background bands. We tested this antibody in endothelial cells. The isoforms were detectable, however, there was a very high level of background bands.
Date published: 2019-06-20
Rated 5 out of 5 by from upenn Gα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
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