Anti-galectin-7 Antibody (D-5) recommended for detection of galectin-7 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-galectin-7 Antibody (D-5): sc-137086

Anti-galectin-7 Antibody (D-5) is rated 5.0 out of 5 by 3.
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Datasheets

    • galectin-7 Antibody (D-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 77-136 mapping at the C-terminus of galectin-7 of human origin
    • recommended for detection of galectin-7 of human origin by WB, IP, IF, IHC(P) and ELISA
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for galectin-7 Antibody (D-5) for WB and IHC(P) applications. These reagents are now offered in bundles with galectin-7 Antibody (D-5) (see ordering information below).
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Can galectin-7 (D-5): sc-137086 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, galectin-7 (D-5): sc-137086 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-05-17
Rated 5 out of 5 by from Strong band detected in WB in human galectin Strong band detected in WB in human galectin-7 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from Robust band in WB in human cervix tissue Robust band in WB in human cervix tissue extract and SCC-4 whole cell lysate. -SCBT QC
Date published: 2015-01-05
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