Anti-galectin-5 Antibody (F-11) recommended for detection of galectin-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-galectin-5 Antibody (F-11): sc-48358

Anti-galectin-5 Antibody (F-11) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-galectin-5 Antibody (F-11) is a mouse monoclonal IgG2a (kappa light chain) galectin-5 antibody provided at 200 µg/ml
    • raised against amino acids 1-145 representing full length galectin-5 of rat origin
    • Anti-galectin-5 Antibody (F-11) is recommended for detection of galectin-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with galectin-9
    • Anti-galectin-5 Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of galectin-5 (F-11): sc-48358.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for galectin-5 Antibody (F-11) for WB and IHC(P) applications. These reagents are now offered in bundles with galectin-5 Antibody (F-11) (see ordering information below).
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What blocking solution should I use with galectin-5 (F-11): sc-48358 monoclonal antibody for Western blot?

Asked by: Cweed
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Good Western blot data of galectin Good Western blot data of galectin-5 expression in rat peripheral blood and rat liver tissue extracts. -SCBT QC
Date published: 2013-01-18
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