Anti-galectin-3 Antibody (M3/38.1.2.8 HL.2) recommended for detection of Galectin-3 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-galectin-3 Antibody (M3/38.1.2.8 HL.2): sc-81728

Anti-galectin-3 Antibody (M3/38.1.2.8 HL.2) is rated 4.0 out of 5 by 1.
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Datasheets

    • galectin-3 Antibody (M3/38.1.2.8 HL.2) is a rat monoclonal IgG2a, cited in 2 publications, provided at 200 µg/ml
    • raised against Galectin-3 of mouse origin
    • recommended for detection of Galectin-3 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See galectin-3 (B2C10): sc-32790 for galectin-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for galectin-3 Antibody (M3/38.1.2.8 HL.2). This work is in progress.
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Can galectin-3 (M3/38.1.2.8 HL.2): sc-81728 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, galectin-3 (M3/38.1.2.8 HL.2): sc-81728 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Strong band seen in WB in non Strong band seen in WB in non-transfected 293T, human galectin-3 transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2013-09-19
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