Anti-galectin-3 Antibody (B2C10) recommended for detection of galectin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-galectin-3 Antibody (B2C10): sc-32790

Anti-galectin-3 Antibody (B2C10) is rated 4.8 out of 5 by 16.
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Datasheets

    • Anti-galectin-3 Antibody (B2C10) is a mouse monoclonal IgG1 κ galectin-3 antibody, cited in 66 publications, provided at 200 µg/ml
    • raised against recombinant Galectin-3 of human origin with an epitope mapping within the first 18 amino acids
    • Anti-galectin-3 Antibody (B2C10) is recommended for detection of galectin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-galectin-3 Antibody (B2C10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also provided azide-free for neutralizing, sc-32790 L, 200 µg/0.1 ml
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of galectin-3 (B2C10): sc-32790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for galectin-3 Antibody (B2C10) for WB and IHC(P) applications. These reagents are now offered in bundles with galectin-3 Antibody (B2C10) (see ordering information below).
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Can galectin-3 Antibody (B2C10): sc-32790 be used to stain 4% PFA fixed, Frozen section of Rat tissue with IF microscopy? Is any antigen retrieval needed in this case?

Asked by: murahman
Thank you for your question. Yes, this antibody is suitable for IF and IHC(P) applications with rat tissue. We always recommend antigen retrieval when fixing with PFA. After fixing the cells, wash in three changes of PBS. Then incubate for 3 minutes in a solution of 0.01% Triton X-100 in PBS. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2019-01-26

中和抗体として使う場合の適用濃度を教えてください。

Asked by: Miya
Thank you for your question. Please refer to PMID: 8634249. Please feel free to contact me if I may be of further assistance. お問い合わせを頂きまして、誠にありがとうございました。ご質問につきまして、PMID 8634249を参照してください。 その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2017-04-12

Can galectin-3 Antibody (B2C10): sc-32790 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, this galectin-3 antibody sc-32790 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 3 out of 5 by from Not working on Zebrafish The antibody works great on human sample. We have tried it also on Zebrafish using the following dilution 1:100 and 1:500, however no signal was detected
Date published: 2021-01-26
Rated 4 out of 5 by from Antibody Review WB I try a trial size sample for this antibody and it works very well for WB at 1:500 dilution in human cells lines lisate. Thanks!
Date published: 2018-11-06
Rated 4 out of 5 by from Antibody review The antibody worked at 1:500 dilution in mouse tissue lysate.
Date published: 2018-06-14
Rated 5 out of 5 by from 清晰的条带 我们在胰腺癌细胞组的wb中使用了这支抗体,有清晰的目的条带,未见杂带,背景清晰,是适合做wb的抗体。
Date published: 2018-04-02
Rated 5 out of 5 by from very good antibody for wb and if im so happy that i recevied a free trial from santacruz.the natibody worked perfectly with wb and if
Date published: 2018-01-02
Rated 5 out of 5 by from Great antibody It worked well. It can detect human Gal3 as well as dog (MDCK cells) gal3.
Date published: 2017-10-20
Rated 5 out of 5 by from worked well for Wb The antibody worked well in the mouse samples and got a pretty good impression of our lab.
Date published: 2017-02-12
Rated 5 out of 5 by from The product is good. galectin-3 antibody the effect is very good. Very nice western blot results, strong signal with low background and the baseband is perfect.
Date published: 2017-02-08
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