Anti-galectin-3 Antibody (B-2) recommended for detection of galectin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-galectin-3 Antibody (B-2): sc-25279

Anti-galectin-3 Antibody (B-2) is rated 5.0 out of 5 by 5.
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Datasheets

    • galectin-3 Antibody (B-2) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 1-160 of galectin-3 of human origin
    • recommended for detection of galectin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See galectin-3 (B2C10): sc-32790 for galectin-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for galectin-3 Antibody (B-2) for WB and IHC(P) applications. These reagents are now offered in bundles with galectin-3 Antibody (B-2) (see ordering information below).
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Can galectin-3 (B-2): sc-25279 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, galectin-3 (B-2): sc-25279 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Published data for IP and IF in human and Published data for IP and IF in human and mouse cell lines: 293T, MCF-7, HeLa, H1299, and MEFs cells. -SCBT Publication Review
Date published: 2015-03-30
Rated 5 out of 5 by from Strong band in WB in human galectin Strong band in WB in human galectin-3 transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from Published data for CoIP in human placental Published data for CoIP in human placental trophoblast cell line BeWo. -SCBT Publication Review
Date published: 2015-03-12
Rated 5 out of 5 by from Published data for WB in human clear cell Published data for WB in human clear cell carcinoma (CCC) of the ovary. -SCBT Publication Review
Date published: 2015-02-24
Rated 5 out of 5 by from Very robust signal seen in WB in A Very robust signal seen in WB in A-431 nuclear extract and Hs 294T, T84 and SW480 whole cell lysates. -SCBT QC
Date published: 2014-04-15
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