Anti-GAL4-TA Antibody (A-2) recommended for detection of GAL4-TA by WB, IP, IF and ELISA

Anti-GAL4-TA Antibody (A-2): sc-46680

Anti-GAL4-TA Antibody (A-2) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-GAL4-TA Antibody (A-2) is a mouse monoclonal IgG1 κ GAL4-TA antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 768-881 mapping within the acidic activator domain of GAL4-TA
    • GAL4-TA Antibody (A-2) is recommended for detection of GAL4-TA by WB, IP, IF and ELISA
    • Anti-GAL4-TA Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GAL4-TA (A-2): sc-46680.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GAL4-TA Antibody (A-2) for WB applications. These reagents are now offered in bundles with GAL4-TA Antibody (A-2) (see ordering information below).
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Can GAL4-TA (A-2): sc-46680 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, GAL4-TA (A-2): sc-46680 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from This antibody worked well for IP This antibody worked well for IP, published in yeast. -SCBT Publication Review
Date published: 2015-01-24
Rated 5 out of 5 by from WB: Single clean band in yeast recombinant GAL4 WB: Single clean band in yeast recombinant GAL4-TA fusion protein. -SCBT QC
Date published: 2013-03-09
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