Gads Antibody (UW40)recommended for detection of Gads of mouse, rat and human origin by WB, IP, IF and ELISA

Gads Antibody (UW40): sc-73652

Gads Antibody (UW40) is rated 3.0 out of 5 by 2.
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Datasheets

    • Gads Antibody (UW40) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against GST-fusion protein expressing Gads of human origin
    • Gads Antibody (UW40) is recommended for detection of Gads of mouse, rat and human origin by WB, IP, IF and ELISA
    • Gads Antibody (UW40) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Gads (UW40): sc-73652.
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what does this antibody bind to? which domain or amino acids of Gads protein this antibody binds?

Asked by: PAPA
Thank you for your question. This antibody was raised against raised against GST-fusion protein expressing Gads of human origin. The exact binding epitope has not been mapped. If you have any further questions, please contact your local distributor.
Answered by: Tech Service
Date published: 2019-03-26

Are there any issues with using sc-73652: Gads (UW40) mouse monoclonal primary antibody on mouse samples?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-73652 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 3 out of 5 by from Working Its working in WB but not a great antibody for this purpose.
Date published: 2018-02-14
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