GABP-β2 siRNA and shRNA Plasmids (h)

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Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Human	GABPB2	126626	1q21.3	XM_017000246, XM_017000247, XM_017000248, XM_017000249, XM_017000250, XM_017000251, XM_017000252, XM_017000253, XM_017000254, NM_001323906, NM_001323907, NM_001323908, NM_001323909, NM_001323910, NM_001323911, NM_001323912, NM_001323913, NM_144618	Q8TAK5	n/a

Description

10 µM, 50-100 transfections
siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
for independent verification of GABP-β2 gene silencing results, individual siRNA duplex components are also available upon request
suitable control antibody: GABP-β1/2 Antibody (E-7): sc-271571
sqRT-PCR Primer also available: GABP-β2 (h)-PR: sc-106219-PR, 20 µl
Get details about si/shRNA Gene Silencers

See Protocol Online.

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Description

20 µl of each primer set is provided at a concentration of 10 µM
GABP-β2 Primer (h) includes A set (forward & reverse) and B set (forward & reverse) primers
Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR

See Protocol Online.

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Description

20 µg, up to 20 transfections
shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
shRNA sequences correspond to GABP-β2 siRNA Gene Silencer sequences
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
shRNA Plasmids are intended only for non-commercial use.
suitable control antibody: GABP-β1/2 Antibody (E-7): sc-271571
sqRT-PCR Primer also available: GABP-β2 (h)-PR: sc-106219-PR, 20 µl

See Protocol Online.

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Description

200 µl viral stock containing 1x 10⁶ infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
shRNA sequences correspond to GABP-β2 siRNA Gene Silencer sequences
After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.
Lentiviral Particles are intended for non-commercial use.

See Protocol Online.

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