GABP-β1/2 Antibody (E-7): sc-271571

5.0(3)

- GABP-β1/2 Antibody (E-7) is a mouse monoclonal IgG_2a κ, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 131-395 mapping at the C-terminus of GABP-β1 of human origin
- GABP- beta 1/2 Antibody (E-7) is recommended for detection of GABP-β1 and GABP-β2 of human origin by WB, IP, IF and ELISA
- Anti-GABP- beta 1/2 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 or Alexa Fluor^® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor^® 680 or Alexa Fluor^® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR^®Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-271571 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GABP-β1/2 (E-7): sc-271571.
- m-IgG Fc BP-HRP, m-IgG_2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GABP-β1/2 Antibody (E-7) for WB applications. These reagents are now offered in bundles with GABP-β1/2 Antibody (E-7) (see ordering information below).

References:

Synergistic transcriptional activation by hGABP and select members of the activation transcription factor/cAMP response element-binding protein family. | Sawada, J., et al. 1999. J Biol Chem. 274: 35475-82. PMID: 10585419
The alpha and beta subunits of the GA-binding protein form a stable heterodimer in solution. Revised model of heterotetrameric complex assembly. | Chinenov, Y., et al. 2000. J Biol Chem. 275: 7749-56. PMID: 10713087
Depolarizing stimulation upregulates GA-binding protein in neurons: a transcription factor involved in the bigenomic expression of cytochrome oxidase subunits. | Zhang, C. and Wong-Riley, MT. 2000. Eur J Neurosci. 12: 1013-23. PMID: 10762332
GA-binding protein alpha/beta is a critical regulator of the BRCA1 promoter. | Atlas, E., et al. 2000. Oncogene. 19: 1933-40. PMID: 10773883
Functional interactions of transcription factor human GA-binding protein subunits. | Suzuki, F., et al. 1998. J Biol Chem. 273: 29302-8. PMID: 9792629
Evolution of the human immunodeficiency virus type 1 long terminal repeat promoter by conversion of an NF-kappaB enhancer element into a GABP binding site. | Verhoef, K., et al. 1999. J Virol. 73: 1331-40. PMID: 9882338

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Human	GABPB1	Entrez Gene ID: 2553	15q21.2	XM_005254274, XM_011521426, XM_017022053, XM_017022054, NM_001320910, NM_001320915, NM_002041, NM_005254, NM_016654, NM_016655, NM_181427	UniProt ID: Q06547	600610
Human	GABPB2	Entrez Gene ID: 126626	1q21.3	XM_017000246, XM_017000247, XM_017000248, XM_017000249, XM_017000250, XM_017000251, XM_017000252, XM_017000253, XM_017000254, NM_001323906, NM_001323907, NM_001323908, NM_001323909, NM_001323910, NM_001323911, NM_001323912, NM_001323913, NM_144618	UniProt ID: Q8TAK5	n/a
Mouse	Gabpb2	Entrez Gene ID: 213054	3 F2.1	XM_006501250, XM_006501251, XM_006501252, XM_006501253, XM_011240058, XM_011240059, XM_011240060, XM_011240061, XM_017319515, NM_029885, NM_172512	UniProt ID: P81069	N/A
Mouse	Gabpb1	Entrez Gene ID: 14391	2 F1	XM_006498755, XM_006498756, XM_006498757, XM_006498758, XM_006498759, XM_006498760, XM_006498761, XM_006498762, XM_006498764, XM_017315588, NM_001271467, NM_001271468, NM_001271469, NM_001271470, NM_001271492, NM_010249, NM_207669	UniProt ID: Q00420	N/A

Full-length GABP-β1/2 protein sequence with immunogen highlighted:

                                        MSLVDLGKKLLEAARAGQDDEVRILMANGAPFTTDWLGTSPLHLAAQYGHYSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHASIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELLIKYGADVHTQSKFCKTAFDISIDNGNEDLAEILQIAMQNQINTNPESPDTVTIHAATPQFIIGPGGVVNLTGLVSSENSSKATDETGVSAVQFGNSSTSVLATLAALAEASAPLSNSSETPVVATEEVVTAESVDGAIQQVVSSGGQQVITIVTDGIQLGNLHSIPTSGIGQPIIVTMPDGQQVLTVPATDIAEETVISEEPPAKRQCIEIIENRVESAEIEEREALQKQLDEANREAQKYRQQLLKKEQEAEAYRQKLEAMTRLQTNKEAV

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GABP-β1/2 Antibody (E-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-GABP-β1/2 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GABP-β1/2 Antibody (E-7) Specifications

Alternate Product Name

GABP-β1/2

Product Clone Name

E-7

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

131-395

Epitope Target

GABP-β1

Epitope Species

human

Product Reactivity/Detection

GABP-β1 and GABP-β2

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

6 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Mouse IgG_2a Binding Proteins

m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

Mouse IgG Kappa Light Chain Binding Proteins

m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin X: TransCruz Reagent

Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-GABP-β1/2 Antibody (E-7) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), ELISA (Enzyme-Linked Immunosorbent Assay).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

What is the recommended fixation method for immunofluorescence staining with GABP-β1/2 (E-7): sc-271571 monoclonal antibody?

Asked by: cjMara

Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining

Answered by: Technical Support

Date published: 2017-02-27

Rated 5 out of 5 by Anon from Working well for WB in HeLa cells Working well for WB in HeLa cells.

Date published: 2015-06-18

Rated 5 out of 5 by HF from Beautiful immunofluorescence nuclear staining Beautiful immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC

Date published: 2015-01-22

Rated 5 out of 5 by MC from Produced positive Western Blot data of GABP Produced positive Western Blot data of GABP- 1/2 expression in Jurkat whole cell lysate and HeLa nuclear extract . -SCBT QC

Date published: 2014-12-14

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GABP-β1/2 Antibody (E-7): sc-271571

QUICK LINKS

SEE ALSO...

References:

GABP-β1/2 Antibody (E-7) Specifications

Alternate Product Name

Product Clone Name

Classification

Clonality

Antibody Isotype

Amino Acids

Epitope Target

Epitope Species

Product Reactivity/Detection

Species Reactivity/Detection

Additional Mammalian Reactivity

Additional Non-Mammalian Reactivity

Applications

Vialing Information

Research Citations

Recommended Secondary Detection Reagents

Available Conjugates/Forms

What is the recommended fixation method for immunofluorescence staining with GABP-β1/2 (E-7): sc-271571 monoclonal antibody?

GABP-β1/2 Antibody (E-7): sc-271571

QUICK LINKS

SEE ALSO...

References:

GABP-β1/2 Antibody (E-7) Specifications

Alternate Product Name

Product Clone Name

Classification

Clonality

Antibody Isotype

Amino Acids

Epitope Target

Epitope Species

Product Reactivity/Detection

Species Reactivity/Detection

Additional Mammalian Reactivity

Additional Non-Mammalian Reactivity

Applications

Vialing Information

Research Citations

Recommended Secondary Detection Reagents

Available Conjugates/Forms

What is the recommended fixation method for immunofluorescence staining with GABP-&beta;1/2 (E-7): sc-271571 monoclonal antibody?

What is the recommended fixation method for immunofluorescence staining with GABP-β1/2 (E-7): sc-271571 monoclonal antibody?