Anti-GABP- beta 1/2 Antibody (E-1) recommended for detection of GABP-β1 and GABP-β2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-GABP-β1/2 Antibody (E-1): sc-271531

Anti-GABP-β1/2 Antibody (E-1) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-GABP-β1/2 Antibody (E-1) is a mouse monoclonal IgG1 κ GABP-β1/2 antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 131-395 mapping at the C-terminus of GABP-β1 of human origin
    • Anti-GABP- beta 1/2 Antibody (E-1) is recommended for detection of GABP-β1 and GABP-β2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and porcine
    • Anti-GABP- beta 1/2 Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-271531 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GABP-β1/2 (E-1): sc-271531.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for GABP-β1/2 Antibody (E-1) for WB applications. This reagent is now offered in a bundle with GABP-β1/2 Antibody (E-1) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with GABP-β1/2 (E-1): sc-271531 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great immunofluorescence nuclear staining Great immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Produced positive Western Blot data of GABP Produced positive Western Blot data of GABP- 1/2 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-04-15
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