Anti-FR Antibody (E-11) recommended for detection of α-FR, β-FR and γ-FR of human origin and α-FR, and β-FR of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA

Anti-FR Antibody (E-11): sc-515521

Anti-FR Antibody (E-11) is rated 5.0 out of 5 by 6.
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Datasheets

    • FR Antibody (E-11) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 210-231 near the C-terminus of FR of human origin
    • FR Antibody (E-11) is recommended for detection of α-FR, β-FR and γ-FR of human origin and α-FR, and β-FR of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-FR Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of FR (E-11): sc-515521.
    • m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for FR Antibody (E-11) for WB and IHC(P) applications. These reagents are now offered in bundles with FR Antibody (E-11) (see ordering information below).
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Can I use this product to detect murine macrophages (raw 264.7) FR using goat anti-mouse secondary antibody?

Asked by: Amir2
Thank you for your question. This FR antibody is recommended for detection in mouse samples. We have not specifically tested the antibody in RAW 264.7. This is a mouse monoclonal IgG2a, and so it can be detected using a secondary antibody that is goat anti-mouse IgG.
Answered by: Technical Support
Date published: 2020-03-19

hi,can you please tell me, FR antibody conjugated with FITC can be useful to find the folate receptors on colon cancer-based spheroids

Asked by: Lokesh
Thank you for your question. We have not tested this antibody (conjugated or unconjugated) for immunofluorescent staining of 3D spheroids.
Answered by: Technical Support
Date published: 2020-03-19

Hi, Can you please tell me what species your FR Antibody (E-11): sc-515521 recognizes? i.e. does it detect human and/or mouse FOLR1? Thanks! JSM

Asked by: JSKM
Thank you for your question. This antibody is reactive with mouse, rat and human.
Answered by: Technical Support
Date published: 2020-03-19

Hi, I have another question about your FR Antibody (E-11): sc-515521. Has it been evaluated for use in flow cytometry? Thanks!

Asked by: JSKM
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2020-12-16

Hi, We intend to procure FR (E-11): sc-515521. Can you please send us a free 10 ug to 20 ug sample? Please reach out to me in the following address. Thank you, Anubhab

Asked by: Anubhab
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2020-03-22

What application is the blocking peptide sc-515521 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2020-03-22
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Rated 5 out of 5 by from KB细胞染色 文献调研KB细胞高表达FR,试验结果也与预期一致,强阳。另外做了人的胎盘、肾脏的染色均为阳性,基本ok
Date published: 2020-06-12
Rated 5 out of 5 by from LE TOP EN A ACHETER UN ET TRES CONTENTE au plaisir de le ravoir
Date published: 2020-04-03
Rated 5 out of 5 by from good antibody This antibody have powerful band detected in a large number of cell lines, good specificity, the analysis results of Western blot were very strong and clear.We are in a very good place in that regard.
Date published: 2017-05-28
Rated 5 out of 5 by from Used to functionalize particles I received a sample of this product and will be ordering a full vial. I used this antibody to functionalize particles in order to target certain cancer cells - particles bound to cells expressing FR and did not bind to non-FR-expressing cells. A great product!
Date published: 2017-05-16
Rated 5 out of 5 by from recommend use it We used Western blot test mosue brain samples, dilution 1:200 effect is obvious, will continue to use this antibody.
Date published: 2017-02-03
Rated 5 out of 5 by from Western blot analysis of FR expression in SHP-77 ( . Western blot analysis of FR expression in SHP-77 (A), A-431 (B), MH-S (C), KNRK (D) and PC-12 (E) whole cell lysates.
Date published: 2017-01-08
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