FOXN1 Antibody (E-3)recommended for detection of FOXN1 of mouse, rat and human origin by WB, IP, IF and ELISA

FOXN1 Antibody (E-3): sc-271256

FOXN1 Antibody (E-3) is rated 4.0 out of 5 by 4.
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Datasheets

    • FOXN1 Antibody (E-3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-270 mapping at the N-terminus of FOXN1 of human origin
    • FOXN1 Antibody (E-3) is recommended for detection of FOXN1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • FOXN1 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-271256 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of FOXN1 (E-3): sc-271256.
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Are there any issues with using sc-271256: FOXN1 (E-3) mouse monoclonal primary antibody on mouse samples?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-271256 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 3 out of 5 by from Partial FOXN1 knockdown, multiple bands I see multiple bands, but intense band at 66kDa. Dharmacon smartpool siFOXN1 partially reduced the intense band. 15µg MCF10A cell lysate, 4-12% gel, 1hr block (5%milk/TBST). Primary antibody,1:500 overnight (5%milk/TBST). Secondary antibody Invitrogen donkey anti mouse 1:500 1hr. Membrane scanned using the Li-cor CLx Odyssey system.
Date published: 2017-02-13
Rated 3 out of 5 by from Good antibody! This antibody is useful for western blot and Immunofluorescence (1:100) of mouse hair follicle,although it needs to try several times.
Date published: 2017-01-13
Rated 5 out of 5 by from FOXN1 抗体 (E-3): sc-271256,荧光,功能不错 FOXN1 抗体 (E-3): sc-271256,使我们的靶基因,从该公司购买此产品后,做小鼠皮肤免疫荧光染色,效果不错,稀释比例为1:100
Date published: 2016-11-29
Rated 5 out of 5 by from Strong band in WB in Raji whole cell lysate and SK Strong band in WB in Raji whole cell lysate and SK-MEL-28 nuclear extract.
Date published: 2014-11-25
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