FOXJ1 Antibody (3-19)recommended for detection of FOXJ1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

FOXJ1 Antibody (3-19): sc-53139

FOXJ1 Antibody (3-19) is rated 4.4 out of 5 by 10.
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Datasheets

    • FOXJ1 Antibody (3-19) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant FOXJ1 of rat origin
    • FOXJ1 Antibody (3-19) is recommended for detection of FOXJ1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • FOXJ1 Antibody (3-19) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of FOXJ1 (3-19): sc-53139.
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If I want to use this antibody for a double stain, how can I do so with only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using a directly conjugated primary antibody. This product, sc-53139, is available conjugated to AF488, AF647, or FITC.
Answered by: Technical Support 4
Date published: 2017-01-17
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Rated 1 out of 5 by from Immunohistochemistry frozen & free floating I tested the antibody for an immunohistochemistry on mouse embryo E12.5, fixed 1h in paraformaldehyde 4%. Dilution: 1/50, Permeabilization solution: PBS 1X- Triton 0,2%(& 0,4%) - Goat serum 10%. It did not work.
Date published: 2017-10-27
Rated 3 out of 5 by from 功能未知 是空管,空管空管空管空管空管空管空管空管空管空管空管空管空管空管空管空管空管空管空管空管,所以无从评价
Date published: 2017-08-15
Rated 5 out of 5 by from Great for Western blot FOXJ1 (3-19) gives a strong band at 58 kDa in Hep G2 and HeLa whole cell lysates for Western blotting (WB).
Date published: 2017-01-23
Rated 5 out of 5 by from Outstanding western data Very clear western data; strong signal with low background
Date published: 2017-01-23
Rated 5 out of 5 by from work well in WB I bought this antibody four month ago,tesed with human cell,and obtained good datas,the antibody is very good
Date published: 2016-12-27
Rated 5 out of 5 by from Published data for IHC in human HAEC cell line Published data for IHC in human HAEC cell line. -SCBT Publication Review
Date published: 2015-04-21
Rated 5 out of 5 by from Robust band in WB in human FOXJ1 transfected Robust band in WB in human FOXJ1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Published IF data in human bronchial tissues Published IF data in human bronchial tissues and airway epithelial cells. -SCBT Publication Review
Date published: 2015-02-08
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