FOXF2 Antibody (L-29)recommended for detection of FOXF2 of mouse, rat and human origin by WB, IP, IF and ELISA

FOXF2 Antibody (L-29): sc-101043

FOXF2 Antibody (L-29) is rated 4.7 out of 5 by 6.
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Datasheets

    • FOXF2 Antibody (L-29) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against recombinant FOXF2 of human origin
    • recommended for detection of FOXF2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of FOXF2 (L-29): sc-101043.
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I am using FOXF2 (L-29): sc-101043 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Weak on skeletal muscle by western blot I tested the antibody at 1:200 by WB on 50ug of muscle samples. I obtained a week band at the expected size.
Date published: 2018-02-12
Rated 5 out of 5 by from Great Nuclear Localization Bright signal seen in IF localized to the nucleus of HeLa cells, good detection also seen with nuclear extracts in Western Blot.
Date published: 2017-02-03
Rated 5 out of 5 by from Great antibody for WB Great antibody for WB; This has worked well in WB with HeLa whole cell lysate.
Date published: 2017-01-15
Rated 5 out of 5 by from Worked well in WB in Hep G2 Worked well in WB in Hep G2, HeLa, A549 and THP-1 nuclear extracts. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Excellent immunofluorescence nuclear staining Excellent immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-22
Rated 5 out of 5 by from Good specificity in WB in HeLa whole cell lysate Good specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-08
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