FOXE3 Antibody (D-6)recommended for detection of FOXE3 of mouse, rat and human origin by WB, IP, IF and ELISA

FOXE3 Antibody (D-6): sc-377465

FOXE3 Antibody (D-6) is rated 5.0 out of 5 by 1.
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Datasheets

    • FOXE3 Antibody (D-6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 232-288 mapping at the C-terminus of FOXE3 of mouse origin
    • recommended for detection of FOXE3 of mouse, rat and human origin by WB, IP, IF and ELISA
    • TransCruz reagent for ChIP application (sc-377465 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Why does the blot show two bands, and neither of which is at 33 kDa (which is the predicted protein size for human FOXE3 protein)?

Asked by: SPadula
Thank you for your question. The Western blot data may not be representative of the endogenous molecular weight for FOXE3. This is detected in a human FOXE3 transfected 293T lysate. The observed molecular weight of FOXE3 may be higher than the predicted molecular weight as a result of post-translational modifications of the protein.
Answered by: Technical Support
Date published: 2019-07-20

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-377465: FOXE3 (D-6) mouse monoclonal antibody will work?

Asked by: jenni
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Works well in WB in mouse FOXP1 transfected Works well in WB in mouse FOXP1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2014-09-30
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