Anti-FMNL2 Antibody (D-3) recommended for detection of FMNL2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-FMNL2 Antibody (D-3): sc-390298

Anti-FMNL2 Antibody (D-3) is rated 4.0 out of 5 by 4.
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Datasheets

    • Anti-FMNL2 Antibody (D-3) is a mouse monoclonal IgG1 (kappa light chain) FMNL2 antibody provided at 200 µg/ml
    • raised against amino acids 461-505 mapping within an internal region of FMNL2 of human origin
    • Anti-FMNL2 Antibody (D-3) is recommended for detection of FMNL2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-FMNL2 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of FMNL2 (D-3): sc-390298.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for FMNL2 Antibody (D-3) for WB and IHC(P) applications. These reagents are now offered in bundles with FMNL2 Antibody (D-3) (see ordering information below).

Can FMNL2 (D-3): sc-390298 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, FMNL2 (D-3): sc-390298 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Ineffective in U2OS cell lines No detectable band was observed when using this antibody with U2OS cell lines.
Date published: 2017-07-31
Rated 5 out of 5 by from Sehr gutes Ergebnis in IP! Hat super funktioniert und hab das schon zwei mal wieder gekauft.
Date published: 2017-05-30
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic staining Beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-05-19
Rated 5 out of 5 by from Nice Western Blot data of FMNL2 expression in IMR Nice Western Blot data of FMNL2 expression in IMR-32 whole cell lysate. -SCBT QC
Date published: 2013-01-01
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