FLVCR Antibody (C-4)recommended for detection of FLVCR of human origin by WB, IP, IF and ELISA

FLVCR Antibody (C-4): sc-390100

FLVCR Antibody (C-4) is rated 5.0 out of 5 by 2.
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Datasheets

    • FLVCR Antibody (C-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-120 mapping at the N-terminus of FLVCR of human origin
    • FLVCR Antibody (C-4) is recommended for detection of FLVCR of human origin by WB, IP, IF and ELISA
    • FLVCR Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of FLVCR (C-4): sc-390100.
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I am using FLVCR (C-4): sc-390100 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Beutiful plasma memebrane staining I got a sample for testing this antibody for IHC. It seems applicable for IHC because the signal was detected in plasma membrane.
Date published: 2017-10-26
Rated 5 out of 5 by from Produced positive Western blot data of FLVCR Produced positive Western blot data of FLVCR expression in HEL 92.1.7 and MIA PaCa-2 whole cell lysates. -SCBT QC
Date published: 2014-09-24
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