FluorescamineAn amine-reacting fluorogenic compound

Fluorescamine (CAS 38183-12-9)

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Synonym: 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3'-dione
Application: An amine-reacting fluorogenic compound
CAS Number: 38183-12-9
Purity: ≥98%
Molecular Weight: 278.26
Molecular Formula: C17H10O4
* Refer to Certificate of Analysis for lot specific data (including water content).
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A non-fluorescent reagent that reacts easily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful in fluorometric assays of amino acids, protein, and proteolytic enzymes. Adequately blocks newly generated amino termini in protein sequence analyses.


References

1. Lai, C.Y. Meth. Enzymol. 47, 236, (1977); 2. Udenfriend, S. Science 178, 871, (1972); 3. Sogowa, K., and Takahaski, K., Use of fluorescamine-labeled casein as a substrate for assay of proteinases. J. Biochem. Tokyo 83, 1783, (1978)

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (partly), ethanol, chloroform, DMSO, and acetonitrile.
Storage :
Store at room temperature
Melting Point :
153-157° C (lit.)
Boiling Point :
~550.3° C at 760 mmHg (Predicted)
Density :
~1.4 g/cm3 (Predicted)
Refractive Index :
n20D 1.69 (Predicted)
Fluoresence :
λex 234 nm; λex 390 nm, λem 475 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
37927
Merck Index :
14: 4158
MDL Number :
MFCD00005928
EC Number :
253-814-5
Beilstein Registry :
921143
SMILES :
C1=CC=C(C=C1)C2=COC3(C2=O)C4=CC=CC=C4C(=O)O3

Download SDS (MSDS)

Certificate of Analysis

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