Anti-Flt-4 Antibody (D-6) recommended for detection of Flt-4 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Flt-4 Antibody (D-6): sc-28297

Anti-Flt-4 Antibody (D-6) is rated 4.6 out of 5 by 8.
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Datasheets

    • Flt-4 Antibody (D-6) is a mouse monoclonal IgG2b κ, cited in 9 publications, provided at 200 µg/ml
    • raised against amino acids 8-240 of Flt-4 of human origin
    • Flt-4 Antibody (D-6) is recommended for detection of Flt-4 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Flt-4 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Flt-4 (D-6): sc-28297.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Flt-4 Antibody (D-6) for WB and IHC(P) applications. These reagents are now offered in bundles with Flt-4 Antibody (D-6) (see ordering information below).
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I am using Flt-4 (D-6): sc-28297 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Gute Ergebnisse IHC-Paraffin Der Antikörper liefert gute Ergebnisse in IHC-Paraffin 1:100
Date published: 2018-02-14
Rated 5 out of 5 by from very good! we use D-6 for IHC in human testis tissue at 1:100 dilution, it worked!
Date published: 2017-06-01
Rated 5 out of 5 by from Sample antibody for WB This antibody sample used for IF,1:300 conceration ,which shows membrance staining.
Date published: 2017-02-12
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic and membrane Great immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-05-19
Rated 4 out of 5 by from Publishable data in ICC using human HEK 293T Publishable data in ICC using human HEK 293T and HepG2 cells, MGZRTcH ESCs, the CCE ESC lines. -SCBT Publication Review
Date published: 2015-03-05
Rated 4 out of 5 by from Publishable data in IHC using human lung Publishable data in IHC using human lung adenocarcinoma cell lines. -SCBT Publication Review
Date published: 2015-02-19
Rated 4 out of 5 by from Robust band detected when using human recombinant Robust band detected when using human recombinant Flt-4. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Various publishable data in WB using the Various publishable data in WB using the following human cell lines: EA. Hy926 and cutaneous melanoma. -SCBT Publication Review
Date published: 2015-02-01
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