Anti-Flg/FGFR1 Antibody (M5G10) recommended for detection of Flg isoforms α and β of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Flg/FGFR1 Antibody (M5G10): sc-57133

Anti-Flg/FGFR1 Antibody (M5G10) is rated 4.5 out of 5 by 2.
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Datasheets

    • Flg/FGFR1 Antibody (M5G10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against the ectodomain of Flg of human origin
    • recommended for detection of Flg isoforms α and β of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See Flg/FGFR1 (M2F12): sc-57132 for Flg/FGFR1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Flg/FGFR1 Antibody (M5G10) for WB and IHC(P) applications. These reagents are now offered in bundles with Flg/FGFR1 Antibody (M5G10) (see ordering information below).

How much sample do you recommend loading on the gel for Western blot detection using Flg (M5G10): sc-57133?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Sharp bands on human VSMCs the sample antibody gives very sharp bands at ~90 kDa, a little bit smaller than that is predicted, and that showed in the datasheet
Date published: 2017-07-20
Rated 4 out of 5 by from Good specificity in WB in human Flg transfected Good specificity in WB in human Flg transfected 293T whole cell lysate. -SCBT QC
Date published: 2013-08-26
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