Anti-Flg/FGFR1 Antibody (F-3) recommended for detection of Flg of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Flg/FGFR1 Antibody (F-3): sc-393911

Anti-Flg/FGFR1 Antibody (F-3) is rated 4.4 out of 5 by 5.
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Datasheets

    • Flg/FGFR1 Antibody (F-3) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 111-122 within an N-terminal extracellular domain of Flg of human origin
    • recommended for detection of Flg of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • See Flg/FGFR1 (M2F12): sc-57132 for Flg/FGFR1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Flg/FGFR1 Antibody (F-3) for WB applications. These reagents are now offered in bundles with Flg/FGFR1 Antibody (F-3) (see ordering information below).
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What application is the blocking peptide sc-393911 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Sharp bands on human VSMCs the sample antibody gives specific, low background bands.
Date published: 2017-07-20
Rated 2 out of 5 by from Average Got multiple bands for FGFR1. Samples were porcine cell lysates.
Date published: 2017-06-16
Rated 5 out of 5 by from Excellent! This antibody is great in western blot analysis of Flg expression in HEK293, HeLa, Jurkat and K-562 whole cell lysates.
Date published: 2017-02-10
Rated 5 out of 5 by from Great Western Blot data of Flg expression Great Western Blot data of Flg expression in HEK293, HeLa, Jurkat and K-562 whole cell lysates. -SCBT QC
Date published: 2015-01-07
Rated 5 out of 5 by from Great Western Blot data of Flg expression in non Great Western Blot data of Flg expression in non-transfected 293T, mouse Flg transfected 293T, WI-38, MOLT-4 and K-562 whole cell lysates. -SCBT QC
Date published: 2014-03-17
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