Fibulin-5 Antibody (3F8D5,7F8D5) recommended for detection of Fibulin-5 of human origin by WB and ELISA

Fibulin-5 Antibody (3F8D5,7F8D5): sc-517203

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Datasheets

    • Fibulin-5 Antibody (3F8D5,7F8D5) is a mouse monoclonal IgM provided at 100 µg/ml
    • raised against amino acids 242-448 of Fibulin-5 of human origin
    • recommended for detection of Fibulin-5 of human origin by WB and ELISA
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How much sample do you recommend loading on the gel for Western blot detection using Fibulin-5 (3F8D5,7F8D5): sc-517203?

Asked by: Dr Ninau Qelp
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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