FcRn Antibody (H-4)recommended for detection of FcRn of mouse, rat and human origin by WB, IP, IF and ELISA

FcRn Antibody (H-4): sc-166413

FcRn Antibody (H-4) is rated 4.0 out of 5 by 3.
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Datasheets

    • FcRn Antibody (H-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 22-276 mapping within an N-terminal extracellular domain of FcRn of mouse origin
    • recommended for detection of FcRn of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See FcRn (B-8): sc-271745 for FcRn antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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what is the recommended conc of FcRn Antibody (H-4): sc-166413 when analysing wt mouse FcRN from homogenised liver tissue?   

Asked by: esben axelgaard
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-166413.pdf If you have any further questions or concerns, please contact our Technical Service, emailing europe@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support Europe
Date published: 2017-09-26

Do you offer a free sample of FcRn Antibody (H-4): sc-166413 ?

Asked by: esben axelgaard
Thank you for your question. Unfortunately we do not offer a sample of sc-166413, our FcRN antibody. However, we do offer a sample of two of our other FcRN antibodies, sc-271745 SAMPLE FcRn (B-8) and sc-393064 SAMPLE FcRn (A-6). If you would like a sample of these, please contact your local distributor or our European office.
Answered by: Tech Service
Date published: 2017-06-02

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-166413: FcRn (H-4) mouse monoclonal antibody will work?

Asked by: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 3 out of 5 by from Different results with every IHC staining Our lab bought this antibody for immunofluorescence on paraformaldehyde fixed tissues and observed variability across different lots.
Date published: 2017-08-07
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-03-23
Rated 5 out of 5 by from neutralization publishable data with rat sample neutralization publishable data with rat sample - SCBT Publication Review
Date published: 2013-07-23
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