FcRn Antibody (B-8)recommended for detection of FcRn of human origin by WB, IP, IF, IHC(P) and ELISA

FcRn Antibody (B-8): sc-271745

FcRn Antibody (B-8) is rated 5.0 out of 5 by 6.
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Datasheets

    • FcRn Antibody (B-8) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 24-297 mapping within an N-terminal extracellular domain of FcRn of human origin
    • FcRn Antibody (B-8) is recommended for detection of FcRn of human origin by WB, IP, IF, IHC(P) and ELISA
    • FcRn Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of FcRn (B-8): sc-271745.
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Is FCRN antibody conjugated with FITC suitable for flow cytometry? If yes, what's the recommended dilution? Thanks.

Asked by: Cordero
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates! We have not yet tested this antibody for flow cytometry. Please check our protocol for our general recommendations for how much antibody to use.
Answered by: Technical Support
Date published: 2019-02-23

Can I use this mouse monoclonal antibody for blocking the FcRn receptor in human cell lines? I pretend block the FcRn receptor during the cell culture (functional studies/inhibition/blocking receptors).

Asked by: Cátia Rocha
Thank you for your question. The FcRn Antibody (B-8): sc-271745 is recommended for detection of FcRn of human origin by WB, IP, IF, IHC(P) and ELISA, but we have no data indicating whether it also blocks the receptor’s function. You can order a free sample of this antibody with your next antibody purchase; we would appreciate your insight.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-271745 PE), fluorescein (sc-271745 FITC), Alexa Fluor® 488 (sc-271745 AF488) or Alexa Fluor® 647 (sc-271745 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-10
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Rated 5 out of 5 by from Outstanding !! Much better product than polyclonal alternatives for multiple applications
Date published: 2017-01-09
Rated 5 out of 5 by from Reliable Antibody for Many Applications This antibody is well-cited for many different common applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal in each application.
Date published: 2016-12-19
Rated 5 out of 5 by from This antibody works well in IF in transfected This antibody works well in IF in transfected human cell lines.
Date published: 2015-10-12
Rated 5 out of 5 by from Crisp band seen in WB using human skeletal Crisp band seen in WB using human skeletal muscle tissue extract. -SCBT QC
Date published: 2015-06-20
Rated 5 out of 5 by from Antibody non Antibody non-specifically binds to human IgG by western blot.
Date published: 2015-03-25
Rated 5 out of 5 by from Publishable data with dendritic cell Publishable data with dendritic cell - SCBT Publication Review
Date published: 2015-03-25
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