FANCL Antibody (H-8)recommended for detection of FANCL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

FANCL Antibody (H-8): sc-137068

FANCL Antibody (H-8) is rated 3.7 out of 5 by 3.
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Datasheets

    • FANCL Antibody (H-8) is a mouse monoclonal mouse IgG2b κ (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 76-375 mapping at the C-terminus of FANCL of human origin
    • recommended for detection of FANCL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can FANCL (H-8): sc-137068 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, FANCL (H-8): sc-137068 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Not good I brought this antibody three months ago to try in breast cancer cell lines but it didn't work at all. I tried ( 1 in 500, TBS without milk) - I didn't get any signal even with long exposure.
Date published: 2017-06-29
Rated 5 out of 5 by from Exceptional immunofluorescence nuclear and Exceptional immunofluorescence nuclear and cytoplasmic staining in formalin-fixed Hep G2 cells. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Strong band in WB in human FANCL transfected Strong band in WB in human FANCL transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-27
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