FANCL Antibody (B-11)recommended for detection of FANCL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

FANCL Antibody (B-11): sc-137067

FANCL Antibody (B-11) is rated 4.9 out of 5 by 7.
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Datasheets

    • FANCL Antibody (B-11) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 76-375 mapping at the C-terminus of FANCL of human origin
    • FANCL Antibody (B-11) is recommended for detection of FANCL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • FANCL Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of FANCL (B-11): sc-137067.
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For FANCL western blot from whole cell lysate what dilution of antibody should be used? Also please tell how long it should be incubated. I used antibody at a dilution of 1:500 and incubated for 4 hours at RT. I am getting multiple specific bands.

Asked by: Gaurav Joshi
Thank you for your question. We recommend using this antibody initially at the starting dilution of 1:200 (dilution range 1:100-1:1,000). We recommend incubating for 1 hour at room temperature in 5% nonfat milk + TBST. Please refer to our protocol online for more detailed information.
Answered by: Technical Support
Date published: 2019-01-31

I am using FANCL (B-11): sc-137067 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent choice for FANCL detection! Great antibody for IHC on paraffin embedded tissue sections with nice clean staining. Also works great for IF.
Date published: 2017-01-31
Rated 4 out of 5 by from Worked well in WB with human samples Worked well in WB with human samples
Date published: 2015-10-12
Rated 5 out of 5 by from Strong band detected in WB in human FANCL Strong band detected in WB in human FANCL transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Robust signal in WB in human FANCL transfected Robust signal in WB in human FANCL transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Published data for IP and WB in Hela cells Published data for IP and WB in Hela cells. -SCBT Publication Review
Date published: 2015-03-19
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic and Excellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-03-16
Rated 5 out of 5 by from Published data for IP and WB in human KSL cells Published data for IP and WB in human KSL cells. -SCBT Publication Review
Date published: 2015-02-03
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