Ezrin Antibody (F-1)recommended for detection of Ezrin of human origin by WB, IP, IF, IHC(P) and ELISA

Ezrin Antibody (F-1): sc-377205

Ezrin Antibody (F-1) is rated 5.0 out of 5 by 5.
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Datasheets
    • Ezrin Antibody (F-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 311-586 of Ezrin of human origin
    • recommended for detection of Ezrin of human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Ezrin (3C12): sc-58758 for Ezrin antibody conjugates, including AC, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
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I am using Ezrin (F-1): sc-377205 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong band see in WB in HeLa and Ramos whole Strong band see in WB in HeLa and Ramos whole cell lysates. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic and Excellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from Positive signal observed in WB in non Positive signal observed in WB in non-transfected 293T, human Ezrin transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Good specificity in WB in Ramos Good specificity in WB in Ramos, MCF7 and K-562 whole cell lysates. -SCBT QC
Date published: 2015-02-02
Rated 5 out of 5 by from Published data for WB in mouse brain Published data for WB in mouse brain. -SCBT Publication Review
Date published: 2015-01-26
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