Ezrin Antibody (6A84)recommended for detection of Ezrin of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)

Ezrin Antibody (6A84): sc-71082

Ezrin Antibody (6A84) is rated 5.0 out of 5 by 3.
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Datasheets
    • Ezrin Antibody (6A84) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 362-585 of Ezrin of human origin
    • recommended for detection of Ezrin of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using Ezrin (6A84): sc-71082 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong positive band observed in WB in A Strong positive band observed in WB in A-431 whole cell lysate. -SCBT QC
Date published: 2015-06-20
Rated 5 out of 5 by from Very robust positive signal observed in WB in non Very robust positive signal observed in WB in non-transfected 293T, human Ezrin transfected 293T and Ramos whole cell lysates. -SCBT QC
Date published: 2015-01-02
Rated 5 out of 5 by from Great specificity in WB in F9 whole cell lysate Great specificity in WB in F9 whole cell lysate. -SCBT QC
Date published: 2014-08-14
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