Ezrin Antibody (5G110)recommended for detection of Ezrin of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)

Ezrin Antibody (5G110): sc-71081

Ezrin Antibody (5G110) is rated 4.0 out of 5 by 3.
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Datasheets
    • Ezrin Antibody (5G110) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 362-585 of Ezrin of human origin
    • recommended for detection of Ezrin of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can Ezrin (5G110): sc-71081 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, Ezrin (5G110): sc-71081 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Strong band of interest observed in WB in A Strong band of interest observed in WB in A-431 whole cell lysate. -SCBT QC
Date published: 2015-07-13
Rated 4 out of 5 by from Good specificity in WB in Jurkat and NIH/3T3 Good specificity in WB in Jurkat and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-05-04
Rated 4 out of 5 by from Robust positive band observed in WB in non Robust positive band observed in WB in non-transfected 293T, human Ezrin transfected 293T and Ramos whole cell lysates. -SCBT QC
Date published: 2014-02-01
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