Ezrin Antibody (4A5)recommended for detection of Ezrin of mouse, rat, human and rabbit origin by WB, IP, IF and IHC(P)

Ezrin Antibody (4A5): sc-32759

Ezrin Antibody (4A5) is rated 4.1 out of 5 by 10.
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Datasheets
    • Ezrin Antibody (4A5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against Ezrin purified from gastric parietal cells of rabbit origin
    • Ezrin Antibody (4A5) is recommended for detection of Ezrin of mouse, rat, human and rabbit origin by WB, IP, IF and IHC(P)
    • Ezrin Antibody (4A5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ezrin (4A5): sc-32759.
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I am using Ezrin (4A5): sc-32759 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Overall very good for western blot This antibody is great for western blot analysis and shows a specific band in mouse cerebellar extracts. When staining mixed primary cerebellar cultures there is no specific staining. The staining appears similar to background seen in the system and therefore appears not to be suitable for primary cerebellar staining. There was no obvious detection in cerebellar Purkinje neurons.
Date published: 2018-01-30
Rated 5 out of 5 by from Nice western blot results in Jurkat We used this antibody for expression detection of Ezrin in western blot. We got a nice band around the correct molecular weight in Jurkat cells.
Date published: 2017-02-06
Rated 4 out of 5 by from Adapted for westernblot on human tissue (one band) Adapted for westernblot on human tissue (one band)
Date published: 2015-10-28
Rated 4 out of 5 by from Published data for WB Published data for WB, IP and IF in rat uterus. -SCBT Publication Review
Date published: 2015-07-09
Rated 4 out of 5 by from Published data for WB and IF in human CD4+ Published data for WB and IF in human CD4+ CXCR4+ CEM cells, Jurkat cell, peripheral blood lymphocytes (PBLs), HeLa P4 cells, and HeLa 243 cells. -SCBT Publication Review
Date published: 2015-07-01
Rated 4 out of 5 by from Published data for WB in human BLM cells Published data for WB in human BLM cells. -SCBT Publication Review
Date published: 2015-05-09
Rated 4 out of 5 by from Published data for WB in human Me Published data for WB in human Me-180 HEC-1-B and VK2/E6E6 cells. -SCBT Publication Review
Date published: 2015-05-08
Rated 4 out of 5 by from Good positive signal observed in WB in Jurkat Good positive signal observed in WB in Jurkat, A-431, HeLa and F9 whole cell lysates. -SCBT QC
Date published: 2015-03-10
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