Ezrin Antibody (3C12)recommended for detection of Ezrin of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)

Ezrin Antibody (3C12): sc-58758

Ezrin Antibody (3C12) is rated 4.8 out of 5 by 17.
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Datasheets
    • Ezrin Antibody (3C12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 362-585 of Ezrin of human origin
    • Ezrin Antibody (3C12) is recommended for detection of Ezrin of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)
    • Ezrin Antibody (3C12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ezrin (3C12): sc-58758.
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For SC58758 AC, I’d like to ask if it conjugate Ezrin antibody through protein A/G, or directly conjugate the antibody? Thanks!

Asked by: Anonymous
Thank you for your question. Our antibodies are covalently bound to the agarose beads via primary amines. The protein G/A/L is covalently linked to the agarose bead.
Answered by: Tech Service
Date published: 2019-08-23

What is the recommended fixation method for IF with Ezrin Antibody (3C12): sc-58758?

Asked by: jenni
We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 4 out of 5 by from Good staining of apical RPE cells of the mouse eye Using a normal staining protocol for IF staining of 10um eye slides from mouse, I got a nicely stained Retinal pigmented epithelium cells.
Date published: 2019-07-17
Rated 3 out of 5 by from FUNCIONA El anticuerpo funciona pero usándolo a grandes concentraciones.
Date published: 2019-06-28
Rated 5 out of 5 by from Highly specific antibody for westernblot Westernblot of cell lysates works very nice and very specific!
Date published: 2018-05-14
Rated 4 out of 5 by from Overall good antibody This antibody works very well for western blot analysis of mouse cerebellar extracts. When probing mixed primary cerebellar cultures, there is varied staining. This appears to have a filament like staining in primary culture, which is likely non-specific background.
Date published: 2018-01-30
Rated 5 out of 5 by from excellent ezrin pattern on the cell surface Cells from a monolayer got stained. However, the cells that did get stained showed excellent ezrin pattern on the cell surface.
Date published: 2017-02-15
Rated 5 out of 5 by from Good Products have a good auxiliary effect, worthy of attention
Date published: 2017-01-10
Rated 5 out of 5 by from Pleased with results Outstanding results for all applications including WB, IF and IHC
Date published: 2017-01-03
Rated 5 out of 5 by from Works with everything! This antibody has great reactivity with a broad range of species. Will satisfy in you WB and do great in your immunostains. It has over 10 citations to support it.
Date published: 2016-12-30
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