Anti-EXOSC7 Antibody (E-12) recommended for detection of EXOSC7 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-EXOSC7 Antibody (E-12): sc-393685

Anti-EXOSC7 Antibody (E-12) is rated 5.0 out of 5 by 1.
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Datasheets

    • EXOSC7 Antibody (E-12) is a mouse monoclonal mouse IgG2a κ (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 133-291 mapping at the C-terminus of EXOSC7 of human origin
    • recommended for detection of EXOSC7 of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for EXOSC7 Antibody (E-12) for WB applications. These reagents are now offered in bundles with EXOSC7 Antibody (E-12) (see ordering information below).
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How much sample do you recommend loading on the gel for Western blot detection using EXOSC7 (E-12): sc-393685?

Asked by: Professor Griffin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Very good Western blot data of EXOSC7 expression Very good Western blot data of EXOSC7 expression in Hep G2, HeLa, Caco-2, A-431 and NCI-H460 whole cell lysates. -SCBT QC
Date published: 2014-02-13
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