Anti-EXOSC10 Antibody (B-8) recommended for detection of EXOSC10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-EXOSC10 Antibody (B-8): sc-374595

Anti-EXOSC10 Antibody (B-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-EXOSC10 Antibody (B-8) is a mouse monoclonal IgG1 κ EXOSC10 antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of EXOSC10 of human origin
    • Anti-EXOSC10 Antibody (B-8) is recommended for detection of EXOSC10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-EXOSC10 Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-374595 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EXOSC10 (B-8): sc-374595.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for EXOSC10 Antibody (B-8) for WB and IHC(P) applications. These reagents are now offered in bundles with EXOSC10 Antibody (B-8) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with EXOSC10 (B-8): sc-374595 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from great features I applied for the samples several days ago and they gave it to me immediately. very good and sensitive.
Date published: 2018-01-31
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of EXOSC10 expression in K-562, NIH/3T3 and HeLa nuclear extracts.
Date published: 2017-01-20
Rated 5 out of 5 by from Exceptional immunofluorescence nucleolar Exceptional immunofluorescence nucleolar staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-29
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