Exo84 Antibody (H-1)recommended for detection of Exo84 of mouse, rat and human origin by WB, IP, IF and ELISA

Exo84 Antibody (H-1): sc-515532

Exo84 Antibody (H-1) is rated 5.0 out of 5 by 2.
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Datasheets

    • Exo84 Antibody (H-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 564-725 mapping near the C-terminus of Exo84 of human origin
    • Exo84 Antibody (H-1) is recommended for detection of Exo84 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Exo84 Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Exo84 (H-1): sc-515532.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with Exo84 (H-1): sc-515532 monoclonal antibody?

Asked by: Trav11
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from an excellent antibody for WB This antibody recognizes a clear and strong band of predicted M.W. on the western blot (1:500 dilution, 40 ug U251 lysate).
Date published: 2017-08-28
Rated 5 out of 5 by from Works well for WB Works well for WB. Used the antibody at 1:1000 dilution for MDA-MB-231 cell lysates.
Date published: 2017-06-07
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