Estrogen Receptor alpha CRISPR Plasmids (h) human Estrogen Receptor alpha-specific CRISPR/Cas9 KO Plasmid, HDR Plasmid, Double Nickase Plasmids, CRISPR Activation Plasmids and Lentiviral Activation Particles

Estrogen Receptor alpha CRISPR Plasmids (h)

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Datasheets

    • Target species: human; for corresponding mouse product, see Estrogen Receptor alpha CRISPR Plasmids (m)
    • CRISPR/Cas9 KO Plasmids consists of Estrogen Receptor alpha-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
    • For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
    • Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
    • Gene knockdown or activation can be assayed using Estrogen Receptor alpha Antibody (F-10): sc-8002
    • All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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hi, we did transfection of human MCF7 cells with ERa CRISPR- KO plasmid sc-400011, I could not see GFP in cells. Does CBh promotor work in these cells?

Asked by: qianwang
Thank you for your question. Please contact our Technical Service Department for help in troubleshooting this CRISPR/Cas9 KO Plasmid. We expect the CBh promotor to work in MCF7 and other commonly transfected cell lines. There may be another issue with your transfection that is preventing the GFP expression from being detected.
Answered by: Technical Support
Date published: 2019-02-23
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