Anti-Estrogen Receptor alpha Antibody (H226) recommended for detection of Estrogen Receptor alpha of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Estrogen Receptor alpha Antibody (H226): sc-53493

Anti-Estrogen Receptor alpha Antibody (H226) is rated 4.7 out of 5 by 6.
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Datasheets

    • Estrogen Receptor alpha Antibody (H226) is a rat monoclonal IgG1, cited in 7 publications, provided at 200 µg/ml
    • raised against Estrogen Receptor alpha of human origin
    • Anti-Estrogen Receptor alpha Antibody (H226) is recommended for detection of Estrogen Receptor alpha of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Estrogen Receptor alpha Antibody (H226) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Estrogen Receptor alpha (H226): sc-53493.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Estrogen Receptor alpha Antibody (H226). This work is in progress.
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Is it possible to get a sample of the ERα Antibody (H226): sc-53493? We would like to test this antibody in mouse brain sections.

Asked by: Takako
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I would like to receive a sample of the ER H226 antibody to test as a replacement for the discontinued HC20. Thank you.

Asked by: warde005
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2021-10-01

Does your company have any test small volume of ER-alpha(H226):sc-53493, ER-alpha (H222):sc-53492, and ER-alpha (2Q418):sc-71064 Abs? If it works in our samples after test, we are going to order more Abs?

Asked by: shlyang
Thank you for the question. We currently offer free Sample sizes for 5 different monoclonal ER alpha antibodies. For clone (2Q418) we currently do not offer a Sample size. Please contact us by email to customercare @ scbt.com and provide your full shipping address and we will be happy to send you available ER alpha antibody Sample sizes.
Answered by: Technical Support Europe
Date published: 2017-12-29

I am using ERα (H226): sc-53493 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great Got good result. Used it to detect ER alpha in Brest cancer tumor section by immunohistochemistry.
Date published: 2018-08-13
Rated 5 out of 5 by from Produced publishable WB and IHC(P) data This ER alpha monocloncal antibody has citations in the literature for the use of WB in rat uterus and femurs and human neuroblastoma cells and IHC(P) data in rat smaples.
Date published: 2017-01-27
Rated 5 out of 5 by from Robust nuclear staining in paraffin embedded Robust nuclear staining in paraffin embedded human breast. -SCBT QC
Date published: 2015-02-26
Rated 4 out of 5 by from Good Western blot analysis of ER expression Good Western blot analysis of ER expression in untreated and Radicicol treated MCF7 whole cell lysates. -SCBT QC
Date published: 2015-01-26
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong ER band detected in MCF7 nuclear extract, and T-47D and ZR-75-1 whole cell lysates under reducing conditions -SCBT QC
Date published: 2015-01-22
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with some background in MCF7 nuclear extract and T-47D whole cell lysate under non-reducing conditions. -SCBT QC
Date published: 2013-12-23
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