Anti-Estrogen Receptor alpha Antibody (H222) recommended for detection of Estrogen Receptor alpha of mouse, rat and human origin by WB

Anti-Estrogen Receptor alpha Antibody (H222): sc-53492

Anti-Estrogen Receptor alpha Antibody (H222) is rated 4.0 out of 5 by 4.
  • y_2022, m_1, d_19, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_3
  • loc_en_US, sid_53492, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 123ms
  • REVIEWS, PRODUCT
loading
5
2
4
1
3
0
2
1
1
0

Datasheets

    • Estrogen Receptor alpha Antibody (H222) is a rat monoclonal IgG1, cited in 7 publications, provided at 200 µg/ml
    • raised against Estrogen Receptor alpha of human origin
    • Estrogen Receptor alpha Antibody (H222) is recommended for detection of Estrogen Receptor alpha of mouse, rat and human origin by WB
    • Anti-Estrogen Receptor alpha Antibody (H222) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Estrogen Receptor alpha (H222): sc-53492.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Estrogen Receptor alpha Antibody (H222). This work is in progress.
loading
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 119ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Rated 2 out of 5 by from Not good for Western blotting, but did work for IP I received a sample of this antibody, which I tested in 2 applications in MCF7 cell lysate: Western blotting and immunoprecipitation. I compared 6 different ERalpha antibodies in my optimization experiments, and this antibody was the only one that did not display any ERalpha (loaded 15 ug lysate) during Western blot visualization. However, when I perform immunoprecipitation with this antibody I see that ERalpha was successfully pulled down.
Date published: 2018-03-08
Rated 5 out of 5 by from Great! This antibody is a great selection for detection of ERα of mouse, rat and human origin by WB
Date published: 2017-01-23
Rated 5 out of 5 by from works well in WB WB in MCF7, a very specific band was observed in ~65 kd
Date published: 2016-12-27
  • y_2022, m_1, d_19, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_3
  • loc_en_US, sid_53492, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT