Estrogen Receptor alpha Antibody (D-12) is a mouse monoclonal IgG2a κ, cited in 260 publications, provided at 200 µg/ml
raised against amino acids 2-185 mapping at the N-terminus of Estrogen Receptor alpha of human origin
Anti-Estrogen Receptor alpha Antibody (D-12) is recommended for detection of Estrogen Receptor alpha of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-Estrogen Receptor alpha Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to TRITC for IF, IHC(P) and FCM
TransCruz reagent for Gel Supershift and ChIP applications (sc-8005 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Estrogen Receptor alpha (D-12): sc-8005.
m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Estrogen Receptor alpha Antibody (D-12) for WB and IHC(P) applications. These reagents are now offered in bundles with Estrogen Receptor alpha Antibody (D-12) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Estrogen Receptor alpha Antibody (D-12) is a high quality monoclonal Estrogen Receptor alpha monoclonal antibody (also designated ER alpha antibody or ERα antibody) suitable for the detection of the protein of mouse, rat and human origin. Estrogen Receptor alpha Antibody (D-12) is available as both the non-conjugated anti-Estrogen Receptor alpha antibody form as well as multiple conjugated forms of anti-Estrogen Receptor alpha antibody, including HRP, FITC, agarose, PE and multiple Alexa Fluor® conjugates. Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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