Anti-ERp72 Antibody (C-7) recommended for detection of ERp72 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-ERp72 Antibody (C-7): sc-376230

Anti-ERp72 Antibody (C-7) is rated 5.0 out of 5 by 5.
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Datasheets

    • ERp72 Antibody (C-7) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 257-528 mapping within an internal region of ERp72 of human origin
    • recommended for detection of ERp72 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for ERp72 Antibody (C-7) for WB and IHC(P) applications. This reagent is now offered in a bundle with ERp72 Antibody (C-7) (see ordering information below).
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Can ERp72 (C-7): sc-376230 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, ERp72 (C-7): sc-376230 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great It works very well in Hs578t and MDA-MB-157 cell lines ( 1:100).
Date published: 2017-10-31
Rated 5 out of 5 by from Strong band observed in WB in Jurkat whole Strong band observed in WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-06-11
Rated 5 out of 5 by from Positive band observed in WB in Caki Positive band observed in WB in Caki-1 whole cell lysate. -SCBT QC
Date published: 2015-06-02
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic and membrane Great immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-02-06
Rated 5 out of 5 by from Band of interest observed in WB in HeLa whole Band of interest observed in WB in HeLa whole cell lysates. -SCBT QC
Date published: 2015-01-23
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