ERp72 Antibody (B-2)recommended for detection of ERp72 of mouse, rat and human origin by WB, IP, IF and ELISA

ERp72 Antibody (B-2): sc-376210

ERp72 Antibody (B-2) is rated 5.0 out of 5 by 4.
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Datasheets
    • ERp72 Antibody (B-2) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 257-528 mapping within an internal region of ERp72 of human origin
    • recommended for detection of ERp72 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with ERp72 (B-2): sc-376210 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Strong band of interest obtained in WB in Caki Strong band of interest obtained in WB in Caki-1 whole cell lysate. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Good antibody for WB in HeLa whole cell lysate Good antibody for WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by from Positive result in WB in Jurkat whole cell lysate Positive result in WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Great immunofluorescence membrane staining Great immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-17
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