ERp57 Antibody (MaP.ERp57) recommended for detection of ERp57 of mouse, rat and human origin by WB, IP, IF and IHC(P)

ERp57 Antibody (MaP.ERp57): sc-23886

ERp57 Antibody (MaP.ERp57) is rated 5.0 out of 5 by 13.
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Datasheets

    • Anti-ERp57 Antibody (MaP.ERp57) is a mouse monoclonal IgG1 (kappa light chain) ERp57 antibody provided at 200 µg/ml
    • raised against recombinant human ERp57
    • Anti-ERp57 Antibody (MaP.ERp57) is recommended for detection of ERp57 of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including canine
    • Anti-ERp57 Antibody (MaP.ERp57) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERp57 (MaP.ERp57): sc-23886.

I am using ERp57 (MaP.ERp57): sc-23886 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good staining works well analysis ERp57 in fixed mouse rain by IF. Antibody gives high signal and low background, Good staining.
Date published: 2017-08-26
Rated 5 out of 5 by from IHC analysis The staining shows reliable on paraffin human skin tissue. Best results was performed for 1:100 antibody dilution , with the IHC staining kit from Dako.
Date published: 2017-07-29
Rated 5 out of 5 by from Very dark band produced in western blot We used this antibody for western blot staining and got a nice western blot at the correct molecular weight in HeLa cells
Date published: 2017-01-27
Rated 5 out of 5 by from nice quality! Distinct band is detected using this product! Nice quality! Got satisfactory results!
Date published: 2016-11-21
Rated 5 out of 5 by from Robust positive signal detected in WB in HeLa Robust positive signal detected in WB in HeLa , Daudi , MDCK and Caki-1 whole cell lysates. -SCBT QC
Date published: 2015-06-02
Rated 5 out of 5 by from Published data for WB and IF in human CD34+ cells Published data for WB and IF in human CD34+ cells. -SCBT Publication Review
Date published: 2015-05-24
Rated 5 out of 5 by from Published data for WB in human epithelial Published data for WB in human epithelial ovarian cancer cell line. -SCBT Publication Review
Date published: 2015-04-09
Rated 5 out of 5 by from Band of interest observed in WB in both non Band of interest observed in WB in both non-transfected 293T and human ERp57 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-10
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